C.__The Penobscot Valley region, including up to 500 acres of land reserved for the Penobscot Nation; and

D.__Washington County and the Downeast region, including up to 500 acres of land reserved for the Passamaquoddy Tribe.

No more than one zone may be established in each of the areas specified in paragraphs A to D, except that the commissioner may designate up to 4 additional zones through the process established in section 5250-L.

2.__Requirements for designation.__The commissioner shall adopt rules establishing the minimum requirements for the designation of Pine Tree Development Zones.__Additionally, each participating unit of local government must agree to maintain at least one prepermitted construction or development site available within the zone on a continual basis throughout the term of the zone.

3.__Limitations.__The designation of Pine Tree Development Zones is subject to the following limitations:

A.__The total area of a zone, including all noncontiguous parcels, may not exceed 5,000 acres;

B.__A zone located in Aroostook County as described in subsection 1, paragraph A may include property that is also included within the Aroostook County Empowerment Zone as designated by the federal Community Renewal Tax Relief Act of 2000, Public Law 106-554;

C.__Pine Tree Development Zone benefits may not be used to encourage or facilitate the transfer of existing positions or property of a qualified business or affiliated businesses into a zone from a location elsewhere in the State;

D.__Pine Tree Development Zone benefits may not be provided based upon any employees or positions transferred by the business or affiliated businesses into a Pine Tree Development Zone from a location elsewhere in the State;

E.__A Pine Tree Development Zone may not consist of more than 20 noncontiguous parcels of property;